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Chinese researchers produce all-aqueous 3D bioprinting technique for regenerative medicine

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Researchers from the University of Drugs, Shenzhen College, China, have designed a Freeform Reconfigurable Embedded All-Liquid (FREAL) bioprinting system to produce 3D architectures microstructures. Through compartmentalized bioink with residing cells, the 3D printed tissue constructs can facilitate medical types, organ-on-chips, so furthering regenerative medicine research. 

“This presents unique alternatives and highly effective equipment since limitless formulations can be intended from between a breadth of purely natural and artificial hydrophilic polymers to mimic tissues,” states a paper published in Sophisticated Elements.

“This printing tactic could be practical to engineer biomimetic, dynamic tissue-like constructs for potential applications in drug screening, in vitro tissue styles, and regenerative drugs.”

Aqueous structures created using FREAL printing. Photo via Shenzhen University.
Aqueous constructions designed utilizing FREAL printing. Picture by using Shenzhen University.

Freeform reconfigurable embedded all-liquid bioprinting

In accordance to the researchers, aqueous or water-centered microstructures are tough to create, handle, and protect as its surfaces are vulnerable to shrink into spherical designs with minimal floor locations. FREAL bioprinting has been developed to advance the bioprinting of sophisticated tissue-like 3D constructs, like arteries, urinary catheters, and tracheae.

In an immiscible aqueous ecosystem, all-liquid microconstructs are formed using aqueous bioinks which capabilities as a biocompatible assistance and pregel solution. Hydrogen bonding interaction is leveraged in FREAL in between polymers in an aqueous two-period procedure (ATPS), which can be stabilized for months. Moreover, distinctive cells can be separately combined with the bioinks and matrices produced to acquire tailor-designed microconstructs with perfusable vascular networks.

Pursuing experimentation, the group noted, “The formulated ATPS allows the continuous producing of all-aqueous 3D constructions and makes certain a sufficient stabilization time versus separation right until the interfacial membrane can be shaped.”

“During printing, if the ink viscosity is also substantial in comparison with that of the matrix, the extruded threads will be dragged by the printhead, comprising the printing fidelity. If the ink viscosity is also lower to suppress the interfacial rigidity result, the printed threads break up rapidly.”

A) A schematic showing the formulation of ATPS. B) FREAL printing by the formulated ATPSs. The ink phase is extruded into a matrix section by translating nozzles with microfluidic types. C) Time-serial optic visuals evaluating the stabilization influence of various ATPS. D) Optical image of tubular slender membranes fashioned by the interfacial hydrogen bonding. E) Optical and fluorescence microscopy photos reveal that both bioinks with hydrodynamic diameters of 30 nm and fluorescent 100 nm polystyrene beads. Photo by means of Shenzhen College.

Accelerating regenerative medication

Residing cells can be right combined into the ink stage or matrix phase in FREAL printing. The scientists deduced that ATPS printing provides a ideal platform for practical cells. “The growth of tissue engineering and regenerative drugs requires the 3D coculture of unique cell strains. By employing a twin-channel microfluidic printhead, distinctive cells with controllable spatial distribution can be printed with each other.

“If the matrix stage is crosslinked, tissue-like constructs in which different cells of controllable compositions and densities are situated in predefined spatial patterns can be fabricated. [However,] we notice that it would be difficult to comprehensively erase exquisite structures even though trying to keep buildings nearby unaffected.”

Aqueous structures produced making use of FREAL printing. Photo via Shenzhen College.

Freeform, Reconfigurable Embedded Printing of All-Aqueous 3D Architectures,” is co-authored by Guanyi Luo, Yafeng Yu, Yuxue Yuan, Xue Chen, Zhou Liu, and Tiantian Kong. 

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Featured picture shows Aqueous constructions created utilizing FREAL printing. Picture by way of Shenzhen College.